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Product Information

Cheek Cell DNA Extraction Kit
Product ID: CHEEK-01
 
 
 
 
 
 
 
 
 
This kit contains supplies to extract DNA isolated from human cheek cells.  Drinking water and isopropyl alcohol must be supplied by the user. 
 
The procedure takes about 5 minutes to do.  There are 4 main steps:
  1. Prepare the saline (using drinking water).
  2. Vigorous rinse your mouth (recovers cheek cells).
  3. Break open (lyse) the cheek cells (using soap provided).
  4. Gently pour cold isopropyl alcohol into tube to form a layer over the soapy cell lysate.
 
If desired, the precipitated DNA can be saved in the extra small tube provided.
Suggested uses:
  • Middle school science classes: as an aid to teaching elementary biology concepts (e.g. cell structure and function; reproduction; adaptation and inherited traits, parts of a cell)
  • Introduction to life sciences
  • Recruitment into biotechnology programs
Plasmid Identification Kit (for Student Assesment in SLCC CE Classes)
Product ID:  PLASM-01
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
Each sample is an uniquely coded unknown, consisting of 2.5 ug of one of three plasmids.  The plasmid concentration is in the range of 100-150 ng/uL.
 
These samples can be used to assess mastery of a variety of biotechnology student learning outcomes, including the following:
  • Successful execution of standard molecular techniques [skills assessment]
    • DNA concentration determination
    • Restriction digest set-up
    • Running gel buffer preparation (concentrated stock and working stock)
    • Agarose gel preparation
    • DNA sample preparation for electrophoresis
    • Gel electrophoresis separation of DNA fragments
  • Application of learned concepts and skills to solve a problem: what is the plasmid? [critical thinking and application assessment]
    • Determination of the appropriate restriction enzyme(s) to use for the digestion(s) necessary to identify the unknown
    • Restriction fragment predictions
    • Appropriate application of the scientific method as well as technical concepts and skills from their “toolbox”
    • Analysis of restriction digest results and determination of the identity of unknown OR determination of best next steps
    • Provision of a data-driven conclusion
  • Proper documentation of experimental results [communication assessment]
    • Correct usage of standard lab report format
    • Accurate communication of work performed
    • Correct usage of technical terms
  • Correct calculation of chemicals needed to prepare reagents (if a.ii buffer is made as concentrated stock) [lab math assessment]
 
Included in the order will be:
 
An instructor’s guide for usage of this product and execution of these assessments is available upon request.
 
Suggested uses:
  • This product is intended ONLY for student assessment within Salt Lake Community College concurrent enrollment biotech classes.
Plasmid Identification Kit (General Use)
Product ID:  PLASM-02
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
Each sample is an uniquely coded unknown, consisting of 2.5 ug of one of three plasmids.  The plasmid concentration is in the range of 100-150 ng/uL.
 
These samples can be used to assess mastery of a variety of biotechnology student learning outcomes, including the following:
  • Successful execution of standard molecular techniques [skills assessment]
    • DNA concentration determination
    • Restriction digest set-up
    • Running gel buffer preparation (concentrated stock and working stock)
    • Agarose gel preparation
    • DNA sample preparation for electrophoresis
    • Gel electrophoresis separation of DNA fragments
  • Application of learned concepts and skills to solve a problem: what is the plasmid? [critical thinking and application assessment]
    • Determination of the appropriate restriction enzyme(s) to use for the digestion(s) necessary to identify the unknown
    • Restriction fragment predictions
    • Appropriate application of the scientific method as well as technical concepts and skills from their “toolbox”
    • Analysis of restriction digest results and determination of the identity of unknown OR determination of best next steps
    • Provision of a data-driven conclusion
  • Proper documentation of experimental results [communication assessment]
    • Correct usage of standard lab report format
    • Accurate communication of work performed
    • Correct usage of technical terms
  • Correct calculation of chemicals needed to prepare reagents (if a.ii buffer is made as concentrated stock) [lab math assessment]
 
Included in the order will be:
  • Instructor's key to the plasmid codes
  • Restriction maps of the three possible plasmids
 
An instructor’s guide for usage of this product and execution of these assessments is available upon request.
 
Suggested uses:
  • Introductory biotechnology lab courses (pre-requisites to advanced courses in which student success is dependent on mastery of the above learning outcomes)
  • Screening for prior experience to waive students out of a course/pre-requisite, or to give students credit