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Lab 4: Restriction Enzyme Digest of H. salsolis DNA

Materials Needed

Kit Items will be provided to you:

*This protocol is optimized for use with restriction enzymes and buffer from New England Biolabs (NEB).  Please order these items directly from NEB; see the H. salsolis Google website for ordering information.  Possible enzymes to use include: SacI-­‐HF, HindIII-­‐HF, and EcoRI-­‐HF.  The buffer and BSA (if needed) will come with the enzyme when ordered.

Please send your order to:

Tonello, Lori [Tonello@neb.com]
Teacher shipping info:
(school) Address

Phone Number

Date to be received